Stimulation of Na+-Ca2+ exchange by purified antibody against alpha-2 repeat of Na+-Ca2+ exchanger in rat cardiomyocytes.
Acta Pharmacol Sin
; 29(10): 1175-80, 2008 Oct.
Article
em En
| MEDLINE
| ID: mdl-18817621
ABSTRACT
AIM:
The aim of the present study was to investigate the effect of the antibody against alpha-2 repeat on Na+-Ca2+ exchanger (NCX) current (I(Na/Ca)). To evaluate the functional specificity of this antibody, its effects on L-type Ca2+ current (I(Ca,L)), voltage-gated Na+ current (I(Na)) and delayed rectifier K+ current (I(K)) were also observed.METHODS:
The whole-cell patch-clamp technique was used in this study.RESULTS:
The antibody against alpha-2 repeat augmented both the outward and inward Na+-Ca2+ exchanger current concentration-dependently, with EC(50) values of 27.9 nmol/L and 24.7 nmol/L, respectively. Meanwhile, the antibody could also increase I(Ca,L) in a concentration-dependent manner with the EC(50) of 33.6 nmol/L. Effects of the antibody on I(Na) and I(K) were not observed in the present study.CONCLUSION:
The present results suggest that antibody against alpha-2 repeat is a stimulating antibody to NCX and could also increase I(Ca,L) in a concentration-dependent manner, but did not have an obvious effect on I(Na) and I(K).
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Sódio
/
Cálcio
/
Anticorpos Bloqueadores
/
Trocador de Sódio e Cálcio
/
Miócitos Cardíacos
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article