Surface plasmon resonance (SPR) analysis of binding interactions of proteins in inner-ear sensory epithelia.
Methods Mol Biol
; 493: 323-43, 2009.
Article
em En
| MEDLINE
| ID: mdl-18839357
ABSTRACT
Surface plasmon resonance is an optical technique utilized for detecting molecular interactions. Binding of a mobile molecule (analyte) to a molecule immobilized on a thin metal film (ligand) changes the refractive index of the film. The angle of extinction of light, reflected after polarized light impinges upon the film, is altered, monitored as a change in detector position for the dip in reflected intensity (the surface plasmon resonance phenomenon). Because the method strictly detects mass, there is no need to label the interacting components, thus eliminating possible changes of their molecular properties. We have utilized surface plasmon resonance to study the interaction of proteins of hair cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas
/
Ressonância de Plasmônio de Superfície
/
Orelha Interna
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article