Normal c-abl gene protein--a nuclear component.
Oncogene
; 6(8): 1459-64, 1991 Aug.
Article
em En
| MEDLINE
| ID: mdl-1886716
ABSTRACT
The subcellular distribution of the c-abl and bcr-abl gene products from KG1A and K562 cells has been studied by two different techniques. Firstly, physical disruption followed by subcellular fractionation was used to demonstrate that normal c-abl (p145) was recovered from the cytosol and the nuclear fractions of KG1A cells. In contrast, bcr-abl products were recovered exclusively from the cytosol fraction of K562 cells. Secondly, indirect immunofluorescence was used to localize c-abl protein to the cytoplasm, nuclear membrane and infrequently to the nucleus of KG1A cells and bcr-abl protein to only the cytoplasm of K562 cells. Thus both the approaches indicate that there is a component of normal c-abl products which appears to be nuclear and this is not reflected in the distribution of the bcr-abl 210 kDa protein, which remains cytosolic.
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Base de dados:
MEDLINE
Assunto principal:
Núcleo Celular
/
Proteínas Proto-Oncogênicas c-abl
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article