Evaluation of functional gene enrichment in a soil metagenomic clone library.
J Microbiol Methods
; 76(1): 105-7, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-18922230
ABSTRACT
We evaluated the use of mixed oligonucleotide probes hybridized to metagenomic clones spotted on high density membranes. The pooled probes included oligonucleotides designed for genes associated with denitrification, antibiotic resistance, and dehalogenation among others. Pyrosequence comparison between the clones and the original DNA demonstrated the utility of clone screening with pooled probes.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Microbiologia do Solo
/
Bactérias
/
Proteínas de Bactérias
/
Biblioteca Gênica
/
Genoma Bacteriano
/
Hibridização de Ácido Nucleico
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article