Chiral separation of raltitrexed by cyclodextrin-modified micellar electrokinetic chromatography.
Anal Bioanal Chem
; 393(1): 321-6, 2009 Jan.
Article
em En
| MEDLINE
| ID: mdl-18931995
ABSTRACT
A rapid and effective method was developed for the chiral separation of raltitrexed (RD) enantiomers by carboxymethyl-beta-cyclodextrin (CM-beta-CD)-modified micellar electrokinetic chromatography (MEKC). Optimization of conditions including the type and concentration of the chiral selector, concentration of sodium dodecyl sulfate (SDS), pH and concentration of the background electrolyte (BGE), capillary temperature, and applied voltage was investigated. The enantiomers of raltitrexed could be separated with satisfactory resolution and linear response by using 75 mM Tris-phosphate at pH 8.0 containing 30 mM SDS and 8 mM CM-beta-CD as buffer system. Furthermore, the usefulness of this method was demonstrated in a purity test of a real synthetic drug sample.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Quinazolinas
/
Tiofenos
/
Cromatografia Capilar Eletrocinética Micelar
/
Beta-Ciclodextrinas
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article