Characterization of Bacillus stearothermophilus infA and of its product IF1.
Gene
; 428(1-2): 31-5, 2009 Jan 01.
Article
em En
| MEDLINE
| ID: mdl-18951960
ABSTRACT
Bacillus stearothermophilus infA encoding translation initiation factor IF1 was cloned and expressed in Escherichia coli and its transcript and protein product characterized. Although the functional properties of B. stearothermophilus and E. coli IF1, compared in several translational tests in the presence of both homologous and heterologous components, are not entirely identical, the two proteins are interchangeable in an in vitro translational system programmed with a natural mRNA. The availability of purified B. stearothermophilus IF1 now allows us to analyze the translation initiation pathway using efficient in vitro tests based entirely on purified components derived from this thermophilic Gram-positive bacterium.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Geobacillus stearothermophilus
/
Proteínas de Bactérias
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Fator de Iniciação 1 em Eucariotos
/
Fator de Iniciação 1 em Procariotos
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article