[Study on aqueous chemical constituents from the flower buds of Eugenia caryophylla].

ABSTRACT

OBJECTIVE: To study the aqueous chemical constituents of the flower buds of Eugenia caryophylla. METHODS: The compounds were isolated by HP-20 resin column chromatography, RP-C18 column chromatography, preparative RP-C18 thin-layer chromatography, preparative RP-C18 high performance liquid chromatography, and their structures were established by NMR and MS evidences. RESULTS: Five compounds were separated from the water extracts. Their structures were identified as quercetin-3-O-glucuronide (1), quercetin-3-O-glucuronide 6"-methyl ester (2), quercetin-3-O-glucopy-ranoside (3) , eugenyl-beta-rutinoside (4) and myricetin (5). CONCLUSION: Compounds 1-4 are isolated from the genus for the first time.