Boundary swapping in the Drosophila Bithorax complex.
Development
; 135(24): 3983-7, 2008 Dec.
Article
em En
| MEDLINE
| ID: mdl-18987027
ABSTRACT
Although the boundary elements of the Drosophila Bithorax complex (BX-C) have properties similar to chromatin insulators, genetic substitution experiments have demonstrated that these elements do more than simply insulate adjacent cis-regulatory domains. Many BX-C boundaries lie between enhancers and their target promoter, and must modulate their activity to allow distal enhancers to communicate with their target promoter. Given this complex function, it is surprising that the numerous BX-C boundaries share little sequence identity. To determine the extent of the similarity between these elements, we tested whether different BX-C boundary elements can functionally substitute for one another. Using gene conversion, we exchanged the Fab-7 and Fab-8 boundaries within the BX-C. Although the Fab-8 boundary can only partially substitute for the Fab-7 boundary, we find that the Fab-7 boundary can almost completely replace the Fab-8 boundary. Our results suggest that although boundary elements are not completely interchangeable, there is a commonality to the mechanism by which boundaries function. This commonality allows different DNA-binding proteins to create functional boundaries.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Genes Homeobox
/
Genes de Insetos
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Proteínas de Homeodomínio
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Proteínas de Drosophila
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Drosophila
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article