[Therapeutic effects of hTR-siRNA adenovirus on human cervical cancer xenografts in nude mice].
Sichuan Da Xue Xue Bao Yi Xue Ban
; 39(5): 711-4, 731, 2008 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-19024296
ABSTRACT
OBJECTIVE:
To study the anti-tumor effects and its mechanism of hTR-siRNA adenovirus on human cervical cancer in vivo.METHODS:
The in vivo model of human cervical cancer was established by the subcutaneous inoculation of HeLa cells at the right armpit of BALB/c nu/nu mice. After successful implantation, the mice were randomized into four groups, in which the mice were intratumorally injected with 0.1 mL of Ad-hTR-siRNA (10(13) pfu/L), or Ad-NT-siRNA (10(13) pfu/L), or Cisplatin (1.20 g/L), or serum-free DMEM alone respectively. These treatments were given once every 3 days for 6 times. After the last injection, the mice were observed for 7 days continuously and sacrificed at the end. The tumors were harvasted, weighed, and sectioned. The TUNEL assay was used to assess the apoptosis of these tumor cells.RESULTS:
Tumors-implanted were established successfully by 100% with HeLa cells. As compared with Ad-NT-siRNA, Ad-hTR-siRNA could slow down tumor growth, decrease tumor volume (45.48%) and tumor weight (34.68%), as well as promote the apoptosis and necrosis of tumor cells. The TUNEL positive cells were about 11.8%. But the anti-tumor activity of Ad-hTR-siRNA didn't catch on Cisplatin's.CONCLUSION:
This study indicated that the hTR-siRNA adenovirus could suppress cervical cancer-xenografted growth in vivo and induce tumor cell apoptosis or necrosis.
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Base de dados:
MEDLINE
Assunto principal:
RNA
/
Terapia Genética
/
Neoplasias do Colo do Útero
/
Adenoviridae
/
Telomerase
/
RNA Interferente Pequeno
Tipo de estudo:
Clinical_trials
Limite:
Animals
/
Female
/
Humans
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article