[Immunosuppression induced by measles virus in adult patients is not related to CD4+ CD25+ regulatory T cell induction].
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi
; 22(3): 211-3, 2008 Jun.
Article
em Zh
| MEDLINE
| ID: mdl-19031706
ABSTRACT
OBJECTIVE:
To investigate of the relationship of the immunosuppression induced by Measles virus in adult patients and CD4+ CD25+ regulatory T cell.METHODS:
Thirty-four patients with measles and 27 healthy control subjects were included in this study. The whole blood was collected and CD4+ CD25+ cell and FoxP3+ cell were analyzed by flow cytometry, and CD4+ CD25- and CD4+ CD25+ T lymphocytes were isolated from PBMCs of patients with measles or healthy donors, CD4+ CD25- T cells were cultured in absence or presence of anti-CD3, or BCG, or live attenuated MV. The cell culture supernatant was collected after 72 hours and the concentration of IFN-gamma and IL-10 was determined.RESULTS:
Compared to healthy donors, we observed a reduction of the number of white blood cells and lymphocytes in patients with measles, but there was not significantly different in the frequency of CD4+ CD25+ T cells and CD4+ CD25high T cells within the total CD4+ population in the blood. Treg from both measles patients and healthy controls significantly inhibited IFN-gamma production by CD4+ CD25- T cells in response to anti-CD3 stimulation.CONCLUSION:
Induction and expansion of Treg may not represent a mechanism involved in the establishment of immune suppression by MV.
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Base de dados:
MEDLINE
Assunto principal:
Ativação Linfocitária
/
Linfócitos T Reguladores
/
Sarampo
/
Vírus do Sarampo
Limite:
Adolescent
/
Adult
/
Female
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2008
Tipo de documento:
Article