A fluorescence sensor for detection of geranyl pyrophosphate by the chemo-ensemble method.
J Org Chem
; 74(2): 895-8, 2009 Jan 16.
Article
em En
| MEDLINE
| ID: mdl-19049370
ABSTRACT
Hexaamide receptor 4 and coumarin phosphate 5 form an ensemble for effective sensing of geranyl pyrophosphate (GPP). Fluorescence resonance energy transfer in the 4 + 5 ensemble diminishes when 5 is replaced by GPP. Receptor 4 binds selectively with GPP over other anions, including fatty acids. The 4-GPP complex in 11 stoichiometry is inferred by NMR and fluorescence titrations. Receptor 4 contains a pseudotetrahedral cleft to accommodate GPP via multiple hydrogen bonds, and the two aliphatic chains exert additional hydrophobic interactions.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Fosfatos de Poli-Isoprenil
/
Corantes Fluorescentes
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article