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Preparation and fractionation of Xenopus laevis egg extracts.
Cross, Marie K; Powers, Maureen.
Afiliação
  • Cross MK; Department of Cell Biology, Emory University, USA. mkcross@emory.edu
J Vis Exp ; (18)2008 Aug 27.
Article em En | MEDLINE | ID: mdl-19066500
Crude and fractionated Xenopus egg extracts can be used to provide ingredients for reconstituting cellular processes for morphological and biochemical analysis. Egg lysis and differential centrifugation are used to prepare the crude extract which in turn in used to prepare fractionated extracts and light membrane preparations.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óvulo / Xenopus laevis / Fracionamento Celular Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Óvulo / Xenopus laevis / Fracionamento Celular Limite: Animals Idioma: En Ano de publicação: 2008 Tipo de documento: Article