Stabilization of D-amino acid oxidase from Rhodosporidium toruloides by immobilization onto magnetic nanoparticles.
Biotechnol Lett
; 31(4): 557-63, 2009 Apr.
Article
em En
| MEDLINE
| ID: mdl-19066733
ABSTRACT
D-amino acid oxidase from Rhodosporidium toruloides was immobilized onto glutaraldehyde-activated magnetic nanoparticles. Approximately four enzyme molecules were attached to one magnetic nanoparticle when the weight ratio of the enzyme to the support was 0.12. After immobilization, the T(m) was increased from 45 degrees C of the free form to 55 degrees C. In the presence of 20 mM H2O2, the immobilized form retained 93% of its activity after 5 h while the free form was completely inactivated after 3.5 h.
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Base de dados:
MEDLINE
Assunto principal:
Basidiomycota
/
Proteínas Fúngicas
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D-Aminoácido Oxidase
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Enzimas Imobilizadas
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Nanopartículas
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Magnetismo
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article