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PCR based RFLP genotyping of bovine lymphocyte antigen DRB3.2 in Iranian Holstein population.
Pashmi, Morteza; Qanbari, Saber; Ghorashi, Seyed Ali; Salehi, Abdolreza.
Afiliação
  • Pashmi M; Animal Genetics Division, National Institute of Genetic Engineering and Biotechnology, Tehran, Iran.
Pak J Biol Sci ; 10(3): 383-7, 2007 Feb 01.
Article em En | MEDLINE | ID: mdl-19069505
ABSTRACT
Major Histocompatibility Complex (MHC) class II locus DRB3 was investigated by PCR based restriction fragment length polymorphism (PCR-RFLP) assay. A total of 262 Holstein cows participating in the national recording system were sampled from 10 herds. A two-step polymerase chain reaction was carried out in order to amplify a 284 base-pair fragment of exon 2 of the target gene. Second PCR products were treated with three restriction endonucleas enzymes RsaI, BstYI and HaeIII. Digested fragments were analyzed by polyacrylamid gel electrophoresis. Twenty-eight BoLA-DRB3 alleles were identified. Identified alleles are BoLA-DRB3.2 *3, *6, *7, *8, *9, *10, *11, *12, *13, *14, *15, *16,20, *21, *22, *23, *24, *25, *26, *27, *28, *32, *36, *37, *40, *51, *iaa and *ibb. The BoLA-DRB3.2*40 allele that was observed in this study has not been reported previously. The calculated frequencies were as follows 2.29, 1.34, 0.19, 14.5, 0.38, 3.05, 12.21, 1.34, 2.29, 1.34, 2.48, 9.16, 0.95, 0.77, 6.68, 9.16, 17.94, 1.15, 0.57, 1.15, 0.95, 0.57, 0.38, 1.91, 0.38, 5.73, 0.19 and 0.95% respectively. The six most frequently observed alleles (BoLA-DRB3.2 *8, *11, *16, *22, *23 and *24) accounted for 69.65% of the alleles in these 10 herds. The results of this study confirm the allelic distribution of six most frequent alleles in Holstein population's worldwide.
Assuntos
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Base de dados: MEDLINE Assunto principal: Polimorfismo de Fragmento de Restrição / Linfócitos / Antígenos HLA-DR Limite: Animals País como assunto: Asia Idioma: En Ano de publicação: 2007 Tipo de documento: Article
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Base de dados: MEDLINE Assunto principal: Polimorfismo de Fragmento de Restrição / Linfócitos / Antígenos HLA-DR Limite: Animals País como assunto: Asia Idioma: En Ano de publicação: 2007 Tipo de documento: Article