Medium- and long-chain 3-hydroxymonocarboxylic acids: analysis by gas chromatography combined with mass spectrometry.
Biomed Chromatogr
; 5(4): 161-4, 1991 Jul.
Article
em En
| MEDLINE
| ID: mdl-1912723
ABSTRACT
Medium- and long-chain 3-hydroxymonocarboxylic acids represent intermediates in the beta-oxidation of fatty acids they accumulate in the plasma of patients with an inherited deficiency of long-chain 3-hydroxyacylcoenzyme A dehydrogenase. 3-Hydroxy acids with chain lengths varying from 6 to 16 were synthesized by a Reformatzky reaction. Capillary gas chromatography of the pertrimethylsilyl derivatives was performed on a CP-Sil 19 CB column, coupled to a quadrupole mass spectrometer in the electron impact mode. Calculation of the retention indices showed that the separation of the 3-hydroxy acids from the homologous straight-chain fatty acids may be troublesome, stressing the need for mass spectrometric identification.
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Base de dados:
MEDLINE
Assunto principal:
Ácidos Carboxílicos
/
Cromatografia Gasosa-Espectrometria de Massas
Limite:
Humans
Idioma:
En
Ano de publicação:
1991
Tipo de documento:
Article