Enhanced antibody responses to Plasmodium falciparum Pfs28 induced in mice by conjugation to ExoProtein A of Pseudomonas aeruginosa with an improved procedure.
Microbes Infect
; 11(3): 408-12, 2009 Mar.
Article
em En
| MEDLINE
| ID: mdl-19146977
ABSTRACT
In this paper we report our efforts to enhance the immunogenicity of Pfs28, a transmission blocking vaccine candidate of Plasmodium falciparum, using a strategy of chemical conjugation. With an improved procedure, Pfs28 was covalently coupled to the mutant and non-toxic ExoProtein A of Pseudomonas aeruginosa by the reaction between thiolated antigen and maleimide modified carrier protein. The optimized process resulted in a higher antigen-carrier conjugation ratio, and the conjugation product could be purified using single-step size-exclusion chromatography. A significant increase in immunogenicity measured by ELISA was observed in mice immunized with conjugated Pfs28 as compared to unconjugated Pfs28.
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Base de dados:
MEDLINE
Assunto principal:
Plasmodium falciparum
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Pseudomonas aeruginosa
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Proteínas de Bactérias
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Vacinas Antimaláricas
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Antígenos de Protozoários
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article