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Molecular detection of human astrovirus in an urban sewage treatment plant in Rio de Janeiro, Brazil.
Guimarães, Flávia Ramos; Ferreira, Fabiana Fioretti Martins; Vieira, Carmen Baur; Fumian, Tulio Machado; Shubo, Tatsuo; Leite, José Paulo Gagliardi; Miagostovich, Marize Pereira.
Afiliação
  • Guimarães FR; Laboratório de Virologia Comparada, Instituto Oswaldo Cruz-Fiocruz, Pavilhão Hélio & Peggy Pereira, Rio de Janeiro, RJ, Brasil.
Mem Inst Oswaldo Cruz ; 103(8): 819-23, 2008 Dec.
Article em En | MEDLINE | ID: mdl-19148423
ABSTRACT
The objective of this study was to evaluate the prevalence and dissemination of human astroviruses (HAstV) in the environment by analyzing urban sewage samples from a wastewater treatment plant in the city of Rio de Janeiro, Brazil. A one-year study was performed with a total of 48 raw and treated sewage composite samples, which were collected biweekly from an activated sludge plant. Virus particles were concentrated by the adsorption-elution method using negatively charged membranes associated to a Centriprep Concentrator 50 (Nihon Millipore). HAstV were detected in 16.7% of the samples in raw and treated sewage by using both qualitative and quantitative reverse transcriptase-polymerase chain reactions (RT-PCR and qPCR, respectively). Positive untreated sewage sample exhibited mean values of 1.1 x 10(4) gEq/mL. The qPCR sensitivity was 18 gEq/reaction. Through utilization of qPCR, a HAstV recovery efficiency of 4.2% and 4.3% was demonstrated for raw and treated sewage samples, respectively. The presence of HAstV in both the raw and treated sewage samples demonstrated the dissemination of these viruses in the environment as well as viral permanence after sewage treatment. There was a reduction in the total and faecal coliform levels, indicating efficiency of the wastewater treatment plant.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mamastrovirus / Esgotos / Microbiologia da Água / Purificação da Água Tipo de estudo: Diagnostic_studies / Qualitative_research / Risk_factors_studies Limite: Humans País como assunto: America do sul / Brasil Idioma: En Ano de publicação: 2008 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Mamastrovirus / Esgotos / Microbiologia da Água / Purificação da Água Tipo de estudo: Diagnostic_studies / Qualitative_research / Risk_factors_studies Limite: Humans País como assunto: America do sul / Brasil Idioma: En Ano de publicação: 2008 Tipo de documento: Article