Enhancement of antibody production from a chicken B cell line DT40 by reducing Pax5 expression.

Magari, Masaki; Aya, Takahiro; Ikeda, Mika; Todo, Kagefumi; Kanayama, Naoki; Ohmori, Hitoshi

Magari, Masaki; Aya, Takahiro; Ikeda, Mika; Todo, Kagefumi; Kanayama, Naoki; Ohmori, Hitoshi.

Afiliação

Magari M; Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okayama University, 3-1-1 Tsushima-Naka, Okayama 700-8530, Japan.

J Biosci Bioeng ; 107(2): 206-9, 2009 Feb.

Article em En | MEDLINE | ID: mdl-19217561

ABSTRACT

ABSTRACT

We developed a novel in vitro antibody (Ab) generation system using a hypermutating chicken B cell line (DT40-SW). We suppressed the expression of the Pax5 transcription factor by targeted disruption of the gene to increase Ab production in isolated clones and produce the desired Abs. This single genetic manipulation resulted in a significant enhancement of Ab production without significantly affecting maximum cell density.

Assuntos

Formação de Anticorpos; Fator de Transcrição PAX5/metabolismo; Animais; Sequência de Bases; Linhagem Celular; Galinhas; Primers do DNA; Fator de Transcrição PAX5/genética; Reação em Cadeia da Polimerase Via Transcriptase Reversa

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Texto completo: 1 Base de dados: MEDLINE Assunto principal: Fator de Transcrição PAX5 / Formação de Anticorpos Limite: Animals Idioma: En Ano de publicação: 2009 Tipo de documento: Article

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