Your browser doesn't support javascript.
loading
Construction and characterization of a high activity mutant of human keratinocyte growth factor-2.
Wang, Jinfeng; Cai, Xin; Zou, Minji; Xu, Tao; Liu, Shen; Wang, Yuanyuan; Wang, Jiaxi; Xu, Donggang.
Afiliação
  • Wang J; Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, 100850 Beijing, China.
Biotechnol Lett ; 31(6): 797-802, 2009 Jun.
Article em En | MEDLINE | ID: mdl-19224135
Keratinocyte growth factor-2 (KGF-2) plays an important role in vertebrate limb development, lung branching morphogenesis, regeneration and reconstruction of the epidermis. Previous studies have used the wild type factor. Here, we have constructed a double-site mutant of human KGF-2, named STEA. STEA possesses higher receptor binding affinity and promotes better proliferation activity on rat tracheal epithelium (RTE) cells than recombinant human KGF-2. These results suggest that the simultaneous mutation of Ser115 to Thr and Glu117 to Ala improves the biological activity of KGF-2.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos e Proteínas de Sinalização Intercelular / Fator 10 de Crescimento de Fibroblastos Limite: Animals / Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Peptídeos e Proteínas de Sinalização Intercelular / Fator 10 de Crescimento de Fibroblastos Limite: Animals / Humans Idioma: En Ano de publicação: 2009 Tipo de documento: Article