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Nitric oxide synthase-dependent responses of the basilar artery during acute infusion of nicotine.
Mayhan, William G; Arrick, Denise M; Sharpe, Glenda M; Sun, Hong.
Afiliação
  • Mayhan WG; Department of Cellular and Integrative Physiology, 985850 Nebraska Medical Center, Omaha, NE 68198-5850, USA. wgmayhan@unmc.edu
Nicotine Tob Res ; 11(3): 270-7, 2009 Mar.
Article em En | MEDLINE | ID: mdl-19246430
INTRODUCTION: Our goals were to determine whether acute exposure to nicotine alters nitric oxide synthase (NOS)-dependent responses of the basilar artery and to identify a potential role for activation of NAD(P)H oxidase in nicotine-induced impairment in NOS-dependent responses of the basilar artery. METHODS: We measured in vivo diameter of the basilar artery in response to NOS-dependent (acetylcholine) and NOS-independent (nitroglycerin) agonists before and during an acute infusion of nicotine (2 microg/kg/min intravenously for 30 min followed by a maintenance dose of 0.35 microg/kg/min). In addition, we measured superoxide anion production (lucigenin chemiluminescence) by the basilar artery in response to nicotine in the absence or presence of apocynin. RESULTS: We found that NOS-dependent, but not NOS-independent, vasodilation was impaired during infusion of nicotine. In addition, treatment of the basilar artery with apocynin (100 microM, 30 min prior to infusion of nicotine) prevented nicotine-induced impairment in NOS-dependent vasodilation. Further, the production of superoxide anion was increased in the basilar artery by nicotine, and this increase could be inhibited by apocynin. DISCUSSION: Our findings suggest that acute exposure to nicotine impairs NOS-dependent dilation of the basilar artery by a mechanism that appears to be related to the release of superoxide anion. A possible source of superoxide may be via the activation of NAD(P)H oxidase.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Artéria Basilar / Acetilcolina / Nitroglicerina / Óxido Nítrico Sintase / Nicotina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Artéria Basilar / Acetilcolina / Nitroglicerina / Óxido Nítrico Sintase / Nicotina Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Ano de publicação: 2009 Tipo de documento: Article