Overexpression of CHMP6 induces cellular oncosis and apoptosis in HeLa cells.
Biosci Biotechnol Biochem
; 73(3): 494-501, 2009 Mar 23.
Article
em En
| MEDLINE
| ID: mdl-19270365
ABSTRACT
Cell death can proceed via at least two distinct pathways, apoptosis and oncosis. Apoptosis is an energy-dependent process characterized morphologically by cell shrinkage, whereas oncosis is defined as a prelethal pathway leading to cell death associated with cellular swelling, organelle swelling, and increased membrane permeability. In this study, we found that overexpression of chromatin modifying protein 6 (CHMP6) induced cell death by a series of experiments, including morphological observation, intracellular ATP determination, caspase-3 activity, and flow cytometry. Typical morphological characteristics consistent with oncosis were observed by transmission electron microscopy. Simultaneously, we obtained some results that indicated apoptosis, but the anti-apoptotic gene Bcl-xL and caspase family inhibitor Z-VAD-FMK had little effect on CHMP6-induced cell death. These results suggest that CHMP6 overexpression can cause cell death, predominantly via oncosis and to a certain extent via apoptosis, and that CHMP6 might be a novel regulator involved in both oncosis and apoptosis.
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Base de dados:
MEDLINE
Assunto principal:
Regulação da Expressão Gênica
/
Apoptose
/
Proteínas de Transporte Vesicular
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article