Troglitazone increases expression of E-cadherin and claudin 4 in human pancreatic cancer cells.
Biochem Biophys Res Commun
; 380(3): 614-9, 2009 Mar 13.
Article
em En
| MEDLINE
| ID: mdl-19285010
ABSTRACT
We examined the effects of troglitazone on expression of E-cadherin and claudin 4 in human pancreatic cancer cells. Troglitazone dose-dependently increased expression of E-cadherin and claudin 4 mRNA and protein in PK-1 cells. Snail, Slug and ZEB1, mRNAs were not changed by troglitazone, indicating that these three transcriptional repressors would not play a role in the induction of E-cadherin by troglitazone. GW9662, a PPARgamma antagonist, failed to block the increased expression of E-cadherin or claudin 4 mRNA, suggesting a PPARgamma-independent pathway. A MEK inhibitor, U0126, increased E-cadherin or claudin 4 mRNA and protein expression, and potently inhibited cell invasion. Because troglitazone down-regulates MEK-ERK signaling and inhibit cell invasion in PK-1 as shown in our previous study, these results suggest that troglitazone increases expression of E-cadherin and claudin 4 possibly through inhibition of MEK-ERK signaling in pancreatic cancer cells, which might be involved in the troglitazone-induced inhibition of cell invasive activity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Neoplasias Pancreáticas
/
Caderinas
/
Cromanos
/
Tiazolidinedionas
/
Proteínas de Membrana
/
Antineoplásicos
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article