A designer biomimetic vector with a chimeric architecture for targeted gene transfer.
J Control Release
; 137(1): 46-53, 2009 Jul 01.
Article
em En
| MEDLINE
| ID: mdl-19303038
Designer biomimetic vectors are genetically engineered biomacromolecules that are designed to mimic viral characteristics in order to overcome the cellular barriers associated with the targeted gene transfer. The vector in this study was genetically engineered to contain at precise locations: a) four tandem repeating units of N-terminal domain of histone H2A to condense DNA into stable nanosize particles suitable for cellular uptake, b) a model targeting motif to target HER2 and enhance internalization of nanoparticles, and c) a pH-responsive synthetic fusogenic peptide to disrupt endosome membranes and promote escape of the nanoparticles into the cytosol. The results demonstrate that a fully functional, multi-domain, designer vector can be engineered to target cells with high specificity, overcome the biological barriers associated with targeted gene transfer, and mediate efficient gene transfer.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Engenharia Genética
/
Técnicas de Transferência de Genes
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Biomimética
/
Vetores Genéticos
Tipo de estudo:
Diagnostic_studies
/
Prognostic_studies
Limite:
Animals
/
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article