[Preliminary study of p53 gene promoter binding site of activator protein-1 in A549 cell lines by chromatin immunoprecipitation].

ABSTRACT

OBJECTIVE: To detect and verify activator protein-1 (AP-1) binding to p53 gene promoter region in A549 cell lines in vitro. METHODS: AP-1 binding to p53 gene promoter region was detected by chromatin immunoprecipitation (ChIP) assay using c-jun specific antibody, and PCR amplified its gene specific DNA fragment. RESULTS: The p53 gene specific fragment was found in the DNA fragment immunoprecipitated by c-jun antibody. CONCLUSION: AP-1 binds to p53 gene promoter region in A549 cells, and regulates its expression.