Seeding-induced self-assembling protein nanowires dramatically increase the sensitivity of immunoassays.
Nano Lett
; 9(6): 2246-50, 2009 Jun.
Article
em En
| MEDLINE
| ID: mdl-19402649
ABSTRACT
Aiming to build a supersensitive and easily operable immunoassay, bifunctional protein nanowires were generated by seeding-induced self-assembling of the yeast amyloid protein Sup35p that genetically fused with protein G and an enzyme (methyl-parathion hydrolase, MPH), respectively. The protein nanowires possessed a high ratio of enzyme molecules to protein G, allowing a dramatic increase of the enzymatic signal when protein G was bound to an antibody target. As a result, a 100-fold enhancement of the sensitivity was obtained when applied in the detection of the Yersinia pestis F1 antigen.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Ensaio de Imunoadsorção Enzimática
/
Nanofios
Tipo de estudo:
Diagnostic_studies
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article