Barley-derived beta-D-glucan induces immunostimulation via a dectin-1-mediated pathway.
Immunol Lett
; 123(2): 144-8, 2009 Apr 27.
Article
em En
| MEDLINE
| ID: mdl-19428562
ABSTRACT
Barley-derived beta-glucan, a linear mixed-linkage beta-glucan composed of 1,3- and 1,4-beta-D-glucopyranose polymers, binds to dectin-1. However, whether it can trigger signal transduction via dectin-1 remains unclear. In this study, we used a reporter gene assay to determine whether barley-derived beta-d-glucan can activate NF-kappaB via dectin-1-mediated signaling when dectin-1 is cotransfected with Syk, CARD9, and Bcl10 in 293T cells. We found that barley-derived beta-D-glucan can activate NF-kappaB leading to cytokine production when dectin-1, Syk, CARD9, and Bcl10 are coexpressed in the cells. We also found that barley-derived beta-D-glucan can induce the phosphorylation of Syk and production of IL-6 in thioglycolate-elicited peritoneal macrophages. These results indicated that the immunostimulatory effects of barley-derived beta-d-glucan might be exerted, at least in part, via dectin-1.
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Base de dados:
MEDLINE
Assunto principal:
Hordeum
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Transdução de Sinais
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NF-kappa B
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Beta-Glucanas
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Proteínas de Membrana
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Proteínas do Tecido Nervoso
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article