Proteomic analysis of naphthalene-induced airway epithelial injury and repair in a cystic fibrosis mouse model.
J Proteome Res
; 8(7): 3606-16, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19438287
ABSTRACT
Combined results from laser capture microdissection of mouse airway epithelial cells followed by high power (MALDI-FTICR) MS, and fluorescent two-dimensional gel elctrophoresis (2D-DIGE) of the whole lung, allowed us to identify proteins differentially expressed after naphthalene induced airway injury. Further, we discovered several novel aspects of Cystic Fibrosis (CF) lung pathology in an F508del-Cftr mouse model using this approach. The combined MALDI-FTICR-MS and 2D-DIGE data show that lung carbonyl reductase (CBR2), involved in prostaglandin metabolism, converting PGE2 to PGF2alpha, is localized to airway cells and is reduced 2-fold in mutant mice compared to normal, both before and after challenge. Further, we observe a downregulation of two key enzymes of retinoic acid metabolism after injury, which is more pronounced in CF mutant mice. These data show that state-of-the-art proteomics can be used to evaluate airway injury in small cell samples. Further, the results suggest the involvement of prostaglandin and retinoic acid metabolism in the abnormal responses of CF mutant mice to injury.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Mucosa Respiratória
/
Fibrose Cística
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Proteômica
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Pulmão
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article