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Uridine insertion/deletion RNA editing in trypanosomatid mitochondria: In search of the editosome.
Osato, Daren; Rogers, Kestrel; Guo, Qiang; Li, Feng; Richmond, Greg; Klug, Felix; Simpson, Larry.
Afiliação
  • Osato D; Department of Microbiology, Immunology and Molecular Genetics, David Geffen School of Medicine, University of California at Los Angeles, Los Angeles, California 90095, USA.
RNA ; 15(7): 1338-44, 2009 Jul.
Article em En | MEDLINE | ID: mdl-19447916
The RNA ligase-containing or L-complex is the core complex involved in uridine insertion/deletion RNA editing in trypanosome mitochondria. Blue native gels of glycerol gradient-separated fractions of mitochondrial lysate from cells transfected with the TAP-tagged editing protein, LC-8 (TbMP44/KREPB5), show a approximately 1 MDa L-complex band and, in addition, two minor higher molecular weight REL1-containing complexes: one (L*a) co-sedimenting with the L-complex and running in the gel at around 1.2 MDa; the other (L*b) showing a continuous increase in molecular weight from 1 MDa to particles sedimenting over 70S. The L*b-complexes appear to be mainly composed of L-complex components, since polypeptide profiles of L- and L*b-complex gradient fractions were similar in composition and L*b-complex bands often degraded to L-complex bands after manipulation or freeze-thaw cycles. The L*a-complex may be artifactual since this gel shift can be produced by various experimental manipulations. However, the nature of the change and any cellular role remain to be determined. The L*b-complexes from both lysate and TAP pull-down were sensitive to RNase A digestion, suggesting that RNA is involved with the stability of the L*b-complexes. The MRP1/2 RNA binding complex is localized mainly in the L*b-complexes in substoichiometric amounts and this association is RNase sensitive. We suggest that the L*b-complexes may provide a scaffold for dynamic interaction with other editing factors during the editing process to form the active holoenzyme or "editosome."
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trypanosoma brucei brucei / Uridina / RNA Mensageiro / Proteínas de Protozoários / RNA de Protozoário / Edição de RNA / Mitocôndrias Limite: Animals Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Trypanosoma brucei brucei / Uridina / RNA Mensageiro / Proteínas de Protozoários / RNA de Protozoário / Edição de RNA / Mitocôndrias Limite: Animals Idioma: En Ano de publicação: 2009 Tipo de documento: Article