Quantum dots as ultrasensitive nanoactuators and sensors of amyloid aggregation in live cells.
J Am Chem Soc
; 131(23): 8102-7, 2009 Jun 17.
Article
em En
| MEDLINE
| ID: mdl-19462994
ABSTRACT
Quantum dots multifunctionalized with the amyloid protein alpha-synuclein act at nanomolar concentrations as very potent inducers of the aggregation of micromolar-millimolar bulk concentrations of the protein in vitro and in cells. Fibrillation in live cells, a process diagnostic of Parkinson's disease, is accelerated up to 15-fold with only approximately 100 nanoparticles. The combination with a tetracysteine-tagged form of alpha-synuclein specific for fluorogenic biarsenicals constitutes a very sensitive system for studying pathological amyloid formation in cells.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Técnicas Biossensoriais
/
Pontos Quânticos
/
Corantes Fluorescentes
/
Amiloide
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article