Reverse transfection using gold nanoparticles.
Methods Mol Biol
; 544: 609-16, 2009.
Article
em En
| MEDLINE
| ID: mdl-19488727
ABSTRACT
Reverse transfection from a solid surface has the potential to deliver genes into various types of cell and tissue more effectively than conventional methods of transfection. We present a method for reverse transfection using a gold colloid (GC) as a nanoscaffold by generating nanoclusters of the DNA/reagent complex on a glass surface, which could then be used for the regulation of the particle size of the complex and delivery of DNA into nuclei. With this method, we have found that the conjugation of gold nanoparticles (20 nm in particle size) to the pEGFP-N1/Jet-PEI complex resulted in an increase in the intensity of fluorescence of enhanced green fluorescent protein (EGFP) (based on the efficiency of transfection) from human mesenchymal stem cells (hMSCs), as compared with the control without GC. In this manner, we constructed a method for reverse transfection using GC to deliver genes into the cells effectively.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Transfecção
/
Coloide de Ouro
/
Nanopartículas Metálicas
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article