Reduced O2 and elevated ROS in sea urchin embryos leads to defects in ectoderm differentiation.
Dev Dyn
; 238(7): 1777-87, 2009 Jul.
Article
em En
| MEDLINE
| ID: mdl-19517573
ABSTRACT
The sea urchin oral-aboral (OA) axis is established in part by Nodal signaling. The OA axis is also influenced by treatments affecting respiration and Nodal transcription is influenced by redox-dependent transcription factors. This suggests that intracellular redox state plays a role in OA axis specification. Since cellular redox state can be altered by the formation of excess reactive oxygen species (ROS), and hypoxia and paraquat generate ROS in cells, we asked whether these treatments affected specification of the OA axis and Nodal expression. Embryos cultured under conditions that elevate ROS, demonstrate perturbed ectoderm specification, but other territories are not affected. Immunohistochemical and Q-RT-PCR analyses revealed that both oral and aboral ectoderm genes are downregulated. Our results argue that elevating ROS in sea urchin embryos by these treatments blocks early steps in ectoderm differentiation preceding the polarization of the ectoderm into oral and aboral territories.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oxigênio
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Ouriços-do-Mar
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Diferenciação Celular
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Espécies Reativas de Oxigênio
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Ectoderma
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article