The relationship between the cusp pattern and plural stem cell compartments in Guinea pig cheek teeth by chasing BrdU-labeling.
Arch Histol Cytol
; 71(5): 317-32, 2008 Dec.
Article
em En
| MEDLINE
| ID: mdl-19556693
ABSTRACT
Continuously growing rodent incisors have a special epithelial structure for maintaining adult stem cells that shows a bulbous epithelial protrusion at the apical end and is referred to as an "apical bud". Guinea pig cheek teeth (premolars and molars), also continuously growing teeth, have a complex crown shape consisting of plural cusps. The present study clarifies the existence of apical buds in guinea pig premolars/molars as it examines the relationship between the crown shape and the orientation of the apical buds by micro-computed tomography (micro-CT) and immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU). One premolar and three molar teeth in each side of the maxillae and mandibles assumed characteristic features each horizontally-sectioned tooth showing a complex zigzag shape was composed of a core of dentin covered by a layer of enamel on all axial surfaces except the buccal of the uppers and the lingual of the lowers. Furthermore, four bulbous epithelial protrusions--including the stellate reticulum--were recognized in the apical end of each tooth, where slow-cycling long-term label-retaining cells resided 20 days after a peritoneal injection of BrdU. These data indicate that guinea pig premolars/molars have four apical buds where the epithelial adult stem cells reside. In contrast, rodent incisors, which show a single cone appearance, are covered by enamel on the labial side and possess only one apical bud. The results of this study suggest that plural apical buds, being arranged bucco-lingually and mesio-distally, produce the crown mold in a zigzag fashion.
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Base de dados:
MEDLINE
Assunto principal:
Coloração e Rotulagem
/
Células-Tronco
/
Bromodesoxiuridina
/
Bochecha
/
Dente Canino
Limite:
Animals
Idioma:
En
Ano de publicação:
2008
Tipo de documento:
Article