Neuronal activity rapidly induces transcription of the CREB-regulated microRNA-132, in vivo.
Hippocampus
; 20(4): 492-8, 2010 Apr.
Article
em En
| MEDLINE
| ID: mdl-19557767
ABSTRACT
Activity-dependent changes in gene-expression are believed to underlie the molecular representation of memory. In this study, we report that in vivo activation of neurons rapidly induces the CREB-regulated microRNA miR-132. To determine if production of miR-132 is regulated by neuronal activity its expression in mouse brain was monitored by quantitative RT-PCR (RT-qPCR). Pilocarpine-induced seizures led to a robust, rapid, and transient increase in the primary transcript of miR-132 (pri-miR-132) followed by a subsequent rise in mature microRNA (miR-132). Activation of neurons in the hippocampus, olfactory bulb, and striatum by contextual fear conditioning, odor-exposure, and cocaine-injection, respectively, also increased pri-miR-132. Induction kinetics of pri-miR-132 were monitored and found to parallel those of immediate early genes, peaking at 45 min and returning to basal levels within 2 h of stimulation. Expression levels of primary and mature-miR-132 increased significantly between postnatal Days 10 and 24. We conclude that miR-132 is an activity-dependent microRNA in vivo, and may contribute to the long-lasting proteomic changes required for experience-dependent neuronal plasticity.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Convulsões
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Transcrição Gênica
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Proteína de Ligação ao Elemento de Resposta ao AMP Cíclico
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MicroRNAs
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Plasticidade Neuronal
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Neurônios
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article