Prevalence and multiplicity of cutaneous beta papilloma viruses in plucked hairs depend on cellular DNA input.
J Virol Methods
; 161(2): 280-3, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19591874
ABSTRACT
In view of the low loads of beta human papillomaviruses in skin samples, amounts of cellular DNA used in qualitative PCR may become limiting for virus detection and introduce variations in prevalence and multiplicity. This issue was explored within the context of a multicentre study and increasing prevalence and multiplicity was found with increasing input amounts of cellular DNA extracted from hair bulbs. To improve the quality and comparability between different epidemiologic studies ideally equal amounts of cellular DNA should be employed. When cellular DNA input varies this should be clearly taken into account in assessing viral prevalence and multiplicity.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
DNA
/
Folículo Piloso
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Infecções por Papillomavirus
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Betapapillomavirus
/
Sobrancelhas
Tipo de estudo:
Diagnostic_studies
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Prevalence_studies
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Qualitative_research
Limite:
Female
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Humans
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Male
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article