[Construction of attenuated Salmonella choleraesuis vaccine strain expressing recombinant antigen of Shiga-like toxin Escherichia coli].
Wei Sheng Wu Xue Bao
; 49(4): 518-23, 2009 Apr.
Article
em Zh
| MEDLINE
| ID: mdl-19621641
ABSTRACT
OBJECTIVE:
To construct an attenuated Salmonella choleraesuis vaccine strain expressing the gene SLT-IIeB and FedF of Shiga-like toxin Escherichia coli (SLTEC) O138 with balanced lethal system.METHODS:
The gene of SLT-IIeB and FedF of SLTEC O138 was amplified, and then recombined with a vector pYA3493 (Asd+). The recombinant was electroporated into attenuated Salmonella choleraesuis C500 (Asd-). The expression of SLT-IIeB and FedF was analyzed by SDS-PAGE. The stability of the vaccine strains was studied by generation culture in vitro.RESULTS:
The stable attenuated Salmonella choleraesuis vaccine strain expressing SLT-IIeB and FedF of SLTEC O138 was constructed with balanced lethal system. The expressed products with protein quality 37000 could react with the antibody of FedF and SLT-IIeB.CONCLUSION:
The attenuated Salmonella choleraesuis vaccine strain could probably serve as a vaccine against edema disease and piglets paratyphoid.
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Base de dados:
MEDLINE
Assunto principal:
Salmonella
/
Vacinas Atenuadas
/
Escherichia coli Shiga Toxigênica
/
Antígenos de Bactérias
Limite:
Animals
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article