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Trailing end-point phenotype antibiotic-sensitive strains of Candida albicans produce different amounts of aspartyl peptidases.
Braga-Silva, L A; Mesquita, D G A; Ribeiro, M D; Carvalho, S M F; Fracalanzza, S E L; Santos, A L S.
Afiliação
  • Braga-Silva LA; Laboratório de Estudos Integrados em Bioquímica Microbiana, Departamento de Microbiologia Geral, Universidade Federal do Rio de Janeiro, Rio de Janeiro, RJ, Brasil.
Braz J Med Biol Res ; 42(8): 765-70, 2009 Aug.
Article em En | MEDLINE | ID: mdl-19649403
Candida albicans is an opportunistic fungal pathogen that causes severe systemic infections in immunosuppressed individuals. C. albicans resistance to antifungal drugs is a severe problem in patients receiving prolonged therapy. Moreover, trailing yeast growth, which is defined as a resistant MIC after 48 h of incubation with triazole antifungal agents but a susceptible MIC after 24 h, has been noted in tests of antifungal susceptibility against some C. albicans isolates. In this context, we recently noticed this phenomenon in our routine susceptibility tests with fluconazole/itraconazole and C. albicans clinical isolates. In the present study, we investigated the production of cell-associated and secreted aspartyl peptidases (Saps) in six trailing clinical isolates of C. albicans, since this class of hydrolytic enzymes is a well-known virulence factor expressed by this fungal pathogen. Sap2, which is the best-studied member of the Sap family, was detected by flow cytometry on the cell surface of yeasts and as a 43-kDa polypeptide in the culture supernatant, as demonstrated by Western blotting assay using an anti-Sap1-3 polyclonal antibody. Released aspartyl peptidase activity was measured with BSA hydrolysis and inhibited by pepstatin A, showing distinct amounts of proteolytic activity ranging from 5.7 (strain 44B) to 133.2 (strain 11) arbitrary units. Taken together, our results showed that trailing clinical isolates of C. albicans produced different amounts of both cellular and secreted aspartyl-type peptidases, suggesting that this phenotypic feature did not generate a regular pattern regarding the expression of Sap.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Candida albicans / Fluconazol / Ácido Aspártico Endopeptidases / Itraconazol / Antifúngicos Tipo de estudo: Diagnostic_studies Limite: Adult / Aged / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2009 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Candida albicans / Fluconazol / Ácido Aspártico Endopeptidases / Itraconazol / Antifúngicos Tipo de estudo: Diagnostic_studies Limite: Adult / Aged / Child, preschool / Female / Humans / Male / Middle aged Idioma: En Ano de publicação: 2009 Tipo de documento: Article