Crystallographic ab initio protein structure solution below atomic resolution.
Nat Methods
; 6(9): 651-3, 2009 Sep.
Article
em En
| MEDLINE
| ID: mdl-19684596
ABSTRACT
Ab initio macromolecular phasing has been so far limited to small proteins diffracting at atomic resolution (beyond 1.2 A) unless heavy atoms are present. We describe a general ab initio phasing method for 2 A data, based on combination of localizing model fragments such as small á-helices with Phaser and density modification with SHELXE. We implemented this approach in the program Arcimboldo to solve a 222-amino-acid structure at 1.95 A.
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Base de dados:
MEDLINE
Assunto principal:
Proteínas
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article