Mapping the interaction site of prion protein and Sho.
Mol Biol Rep
; 37(5): 2295-300, 2010 Jun.
Article
em En
| MEDLINE
| ID: mdl-19685161
ABSTRACT
The cellular prion protein (PrP(C)) is a highly conserved protein among mammals and is considered to have important cellular functions. Despite decades of intensive research, however, the physiological function of PrP(C) remains unclear. Sho (Shadoo, shadow of prion protein) and PrP(C) have similar N-terminals, which suggests that the two proteins share biological functions. Using truncation mutants of both proteins and yeast two-hybrid analysis, with validation by co-immunoprecipitation and surface plasmon resonance (SPR), we have identified an interaction between Sho 61-77 and PrP(C) 108-126 domains. This indicates that Sho may play a role in the physiological function of PrP(C) and prion pathogenesis.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Príons
/
Mapeamento de Interação de Proteínas
/
Proteínas do Tecido Nervoso
Tipo de estudo:
Prognostic_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article