Purification and characterisation of proteins with cardiac stimulatory and haemolytic activity from the anemone Actinia tenebrosa.
Toxicon
; 28(1): 29-41, 1990.
Article
em En
| MEDLINE
| ID: mdl-1970442
ABSTRACT
Three new proteins with cardiac stimulatory and haemolytic activity, designated tenebrosins-A, -B and -C, have been purified from the Australian sea anemone Actinia tenebrosa. These proteins are basic (pI greater than or equal to 9.4), have mol. wt of about 20,000, and have very similar amino acid compositions and N-terminal amino acid sequences. None of the proteins contains cysteine or cystine residues. On isolated, spontaneously beating guinea pig atria they exhibit at 1-2 nM strong positive inotropic and slight to moderate chronotropic effects. In some cases a transient negative inotropic effect occurs prior to onset of the positive inotropic response. The proteins are also haemolytic, producing 50% haemolysis of guinea pig erythrocytes at concentrations similar to those showing positive inotropic effects.
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Base de dados:
MEDLINE
Assunto principal:
Cardiotônicos
/
Proteínas
/
Cnidários
/
Venenos de Cnidários
/
Hemólise
Limite:
Animals
País como assunto:
Oceania
Idioma:
En
Ano de publicação:
1990
Tipo de documento:
Article