Separation of roles of Zip1 in meiosis revealed in heterozygous mutants of Saccharomyces cerevisiae.
Mol Genet Genomics
; 282(5): 453-62, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19714362
ABSTRACT
Synapsis of homologs during meiotic prophase I is associated with a protein complex built along the bivalents--the synaptonemal complex (SC). Mutations in the SC-component gene ZIP1 diminish SC formation, leading to reduced recombination levels and low spore viability. Here we show that in SK1 strains heterozygous for a deletion of ZIP1 in certain regions meiotic interference are impaired with no decrease in recombination levels. The extent of synapsis is over all reduced and NDJ levels of a large endogenous chromosome and of artificial chromosomes (YACs) rise to twice the level of wild type strains. A substantial proportion of mis-segregating YACs had undergone crossing over. This demonstrates that different functions of Zip1 display differential sensitivities to changes in expression levels.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
/
Proteínas Nucleares
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Proteínas de Saccharomyces cerevisiae
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Heterozigoto
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Meiose
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Mutação
Limite:
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article