The hinge region fragment of immunoglobulin G improves immunogenicity of recombinant gonadotrophin-releasing hormone conjugated to the T-helper epitope in designing peptide vaccines.
Immunology
; 128(1 Suppl): e506-13, 2009 Sep.
Article
em En
| MEDLINE
| ID: mdl-19740311
ABSTRACT
In our previous study, the hinge fragment (225-232/225'-232') of human immunoglobulin G1 (IgG1) was used as a space peptide linker for synthesizing the GnRH3-hinge-MVP chimeric peptide, whereby three repeated gonadotrophin-releasing hormone (GnRH) units and a T-cell epitope from measles virus fusion protein (MVP) were amide-bond-linked at the N and C terminus, respectively, to the hinge peptide for producing anti-GnRH antibody responses. To investigate whether or not the hinge region fragment can improve the immunogenicity of GnRH, we further synthesized and purified GnRH3-hinge-MVP, GnRH3-hinge and GnRH3-MVP using recombinant DNA technology. Under high pH conditions, GnRH3-hinge-MVP was capable of forming double-chain structures. Immunization of male mice with the immunogens of GnRH3-hinge-MVP resulted in the generation of high-titre antibodies specific for GnRH. The synthetic GnRH3-hinge and GnRH3-MVP induced a lower titre of anti-GnRH antibody than GnRH3-hinge-MVP. This was followed by a decrease in serum testosterone levels, which resulted in a low level of expression of the relaxin-like factor gene in the testis. Our data suggest that peptide and T-cell epitopes oriented at the N-terminus or C-terminus of hinge peptides simplify the antigenic peptide conjugates and may be considered as potential synthetic immunogens.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Proteínas Virais
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Hormônio Liberador de Gonadotropina
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Epitopos de Linfócito T
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Vacinas de Subunidades Antigênicas
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article