Quantitative miRNA expression analysis: comparing microarrays with next-generation sequencing.
RNA
; 15(11): 2028-34, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19745027
ABSTRACT
Recently, next-generation sequencing has been introduced as a promising, new platform for assessing the copy number of transcripts, while the existing microarray technology is considered less reliable for absolute, quantitative expression measurements. Nonetheless, so far, results from the two technologies have only been compared based on biological data, leading to the conclusion that, although they are somewhat correlated, expression values differ significantly. Here, we use synthetic RNA samples, resembling human microRNA samples, to find that microarray expression measures actually correlate better with sample RNA content than expression measures obtained from sequencing data. In addition, microarrays appear highly sensitive and perform equivalently to next-generation sequencing in terms of reproducibility and relative ratio quantification.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Análise de Sequência de RNA
/
Análise de Sequência com Séries de Oligonucleotídeos
/
MicroRNAs
Tipo de estudo:
Evaluation_studies
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article