[Relationship between glucocorticoid receptors and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura].
Zhongguo Dang Dai Er Ke Za Zhi
; 11(9): 714-6, 2009 Sep.
Article
em Zh
| MEDLINE
| ID: mdl-19755017
OBJECTIVE: To identify the relationship between the expression of alpha and beta-isoforms of glucocorticoid receptors (GR) in peripheral blood mononuclear cells (PBMC) and glucocorticoid resistance in children with idiopathic thrombocytopenia purpura (ITP). METHODS: Real-time PCR was used to detect the expression of GR alpha and GR beta mRNA in PBMC from 30 children with ITP (glucocorticoid-sensitive, n=18; glucocorticoid-resistant, n=12) and 10 healthy children (control group). Enzyme immunoassay was used to measure plasma levels of total glucocorticoids. RESULTS: There were no significant differences in PBMC GR alpha mRNA levels among the glucocorticoid sensitive, the glucocorticoid-resistant and the control groups. Compared with the glucocorticoid-sensitive and the control groups, the expression of GR beta mRNA in the glucocorticoid-resistant group was significantly up-regulated (p<0.01). Plasma total glucocorticoids level in the glucocorticoid-resistant group was found to be much higher than that in the glucocorticoid-sensitive and the control groups (p<0.01). CONCLUSIONS: The up-regulated expression of GR beta mRNA may associated with glucocorticoid resistance in children with ITP.
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Base de dados:
MEDLINE
Assunto principal:
Receptores de Glucocorticoides
/
Púrpura Trombocitopênica Idiopática
/
Glucocorticoides
Limite:
Child
/
Child, preschool
/
Female
/
Humans
/
Male
Idioma:
Zh
Ano de publicação:
2009
Tipo de documento:
Article