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Expression, purification and characterization of recombinant human interleukin-22 in Pichia pastoris.
Cai, Xin; Wang, Jinfeng; Wang, Yuanyuan; Yang, Yongchang; Gao, Jie; Fu, Wenliang; Wang, Jiaxi; Xu, Donggang.
Afiliação
  • Cai X; Laboratory of Molecular Genetics, Beijing Institute of Basic Medical Sciences, 100850 Beijing, China.
Mol Biol Rep ; 37(6): 2609-13, 2010 Jul.
Article em En | MEDLINE | ID: mdl-19760099
Interleukin-22 (IL-22) is a member of the IL-10 family. Its potential in clinical use has been highlighted for its important roles in promoting antimicrobial defense and preventing epithelial damages. Previous studies have reported that IL-22 can be expressed using prokaryotic systems and purified from inclusion bodies, however the recovery rate was poor. To produce functional IL-22 with a high yield, human IL-22 was inserted into the eukaryotic expression vector pPICZalphaA and transformed into Pichia pastoris. The expression of recombinant human IL-22 (rhIL-22) was induced by methanol and accounted for about 85% of the total secreted proteins. A simple purification strategy was established to purify the rhIL-22 from the culture supernatant, yielding 100 mg/l at 90% purity by chromatography with a SP Sepharose FF column. Bioactivity analysis showed the purified rhIL-22 demonstrated a specific activity that was comparable with the commercial one. This study provides a new strategy for large-scale production of bioactive IL-22 for use in basic studies and therapeutic applications.
Assuntos

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pichia / Proteínas Recombinantes / Interleucinas Limite: Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article

Texto completo: 1 Base de dados: MEDLINE Assunto principal: Pichia / Proteínas Recombinantes / Interleucinas Limite: Humans Idioma: En Ano de publicação: 2010 Tipo de documento: Article