Yeast RNase III triggers polyadenylation-independent transcription termination.
Mol Cell
; 36(1): 99-109, 2009 Oct 09.
Article
em En
| MEDLINE
| ID: mdl-19818713
ABSTRACT
Transcription termination of messenger RNA (mRNA) is normally achieved by polyadenylation followed by Rat1p-dependent 5'-3' exoribonuleolytic degradation of the downstream transcript. Here we show that the yeast ortholog of the dsRNA-specific ribonuclease III (Rnt1p) may trigger Rat1p-dependent termination of RNA transcripts that fail to terminate near polyadenylation signals. Rnt1p cleavage sites were found downstream of several genes, and the deletion of RNT1 resulted in transcription readthrough. Inactivation of Rat1p impaired Rnt1p-dependent termination and resulted in the accumulation of 3' end cleavage products. These results support a model for transcription termination in which cotranscriptional cleavage by Rnt1p provides access for exoribonucleases in the absence of polyadenylation signals.
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Base de dados:
MEDLINE
Assunto principal:
Saccharomyces cerevisiae
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Transcrição Gênica
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Regiões Terminadoras Genéticas
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Proteínas de Saccharomyces cerevisiae
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Ribonuclease III
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article