Development of ELISAs for the class-specific determination of organophosphorus pesticides.
J Agric Food Chem
; 57(21): 10004-13, 2009 Nov 11.
Article
em En
| MEDLINE
| ID: mdl-19839635
ABSTRACT
Enzyme-linked immunosorbent assays (ELISAs) for the class-specific determination of organophosphorus (OP) pesticides were developed from monoclonal antibodies raised against haptens with the functional group common to OP pesticides. To develop antigen-coated, indirect, competitive ELISAs, four haptens with different spacer arm structures were used to prepare antibodies, while eight haptens were tested for use as coating antigens. A total of 32 ELISAs were developed with one selected as the most suitable one based on average IC(50) and % CV values. The chosen ELISA showed class-selective response to O,O-diethyl phosphorothioate and phosphorodithioate OP pesticides with negligible cross-reactivity to other types of pesticides. Average IC(50) and % CV values of this ELISA for the 12 OP pesticides were 89 ng/mL and 96%, respectively. Compared to ELISAs previously developed with the same objective, the current ELISA demonstrates better sensitivity based on much lower mean IC(50) values in addition to improved class-selective determination based on considerably lower % CV values as well as precise discrimination against other types of pesticides.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Compostos Organofosforados
/
Praguicidas
/
Ensaio de Imunoadsorção Enzimática
Tipo de estudo:
Evaluation_studies
Limite:
Animals
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article