Use of ion mobility mass spectrometry and a collision cross-section algorithm to study an organometallic ruthenium anticancer complex and its adducts with a DNA oligonucleotide.
Rapid Commun Mass Spectrom
; 23(22): 3563-9, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19844963
ABSTRACT
We report the development of an enhanced algorithm for the calculation of collision cross-sections in combination with Travelling-Wave ion mobility mass spectrometry technology and its optimisation and evaluation through the analysis of an organoruthenium anticancer complex [(eta6-biphenyl)Ru(II)(en)Cl]+. Excellent agreement was obtained between the experimentally determined and theoretically determined collision cross-sections of the complex and its major product ion formed via collision-induced dissociation. Collision cross-sections were also experimentally determined for adducts of this ruthenium complex with the single-stranded oligonucleotide hexamer d(CACGTG). Ion mobility tandem mass spectrometry measurements have allowed the binding sites for ruthenium on the oligonucleotide to be determined.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Oligonucleotídeos
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Compostos Organometálicos
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Espectrometria de Massas
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Compostos de Rutênio
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Adutos de DNA
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Antineoplásicos
Tipo de estudo:
Evaluation_studies
/
Prognostic_studies
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article