Syndecan-2 functions as a docking receptor for pro-matrix metalloproteinase-7 in human colon cancer cells.
J Biol Chem
; 284(51): 35692-701, 2009 Dec 18.
Article
em En
| MEDLINE
| ID: mdl-19858218
ABSTRACT
Although elevated syndecan-2 expression is known to be crucial for the tumorigenic activity in colon carcinoma cells, how syndecan-2 regulates colon cancer is unclear. In human colon adenocarcinoma tissue samples, we found that both mRNA and protein expression of syndecan-2 were increased, compared with the neighboring normal epithelium, suggesting that syndecan-2 plays functional roles in human colon cancer cells. Consistent with this notion, syndecan-2-overexpressing HT-29 colon adenocarcinoma cells showed enhanced migration/invasion, anchorage-independent growth, and primary tumor formation in nude mice, paralleling their morphological changes into highly tumorigenic cells. In addition, our experiments revealed that syndecan-2 enhanced both expression and secretion of matrix metalloproteinase-7 (MMP-7), directly interacted with pro-MMP-7, and potentiated the enzymatic activity of pro-MMP-7 by activating its processing into the active MMP-7. Collectively, these data strongly suggest that syndecan-2 functions as a docking receptor for pro-MMP-7 in colon cancer cells.
Texto completo:
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Base de dados:
MEDLINE
Assunto principal:
Adenocarcinoma
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Movimento Celular
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Neoplasias do Colo
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Metaloproteinase 7 da Matriz
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Sindecana-2
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Proteínas de Neoplasias
Limite:
Animals
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Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article