GLS/IL-12-modified Mycobacterium smegmatis as a novel anti-tuberculosis immunotherapeutic vaccine.
Int J Tuberc Lung Dis
; 13(11): 1360-6, 2009 Nov.
Article
em En
| MEDLINE
| ID: mdl-19861007
ABSTRACT
OBJECTIVE:
To study the effects and mechanisms of recombinant Mycobacterium smegmatis (rMS) carrying pZM03 (a co-expression plasmid encoding human granulysin [GLS] and murine interleukin 12 [IL-12]) on murine M. tuberculosis infection.DESIGN:
BALB/c mice infected with M. tuberculosis were treated with normal saline, M. smegmatis, pZM03 or rMS. The number of viable bacteria in the lungs and spleens were counted to observe the therapeutic effects. The levels of IL-12 and interferon-gamma (IFN-gamma) in serum, and IFN-gamma and tumour necrosis factor-alpha (TNF-alpha) released from spleen lymphocytes were detected to observe the T-helper 1 (Th1) response. Secretory IgA (SIgA) in bronchoalveolar lavage fluid was measured to observe the mucosal immunity. The lungs and spleens were prepared for pathological analysis.RESULTS:
The rMS group showed a significantly reduced number of colony-forming units compared to the other groups. The expression of GLS in the tissue, and increased levels of IL-12, IFN-gamma, TNF-alpha and SIgA, were found in the rMS group. The pathological changes in the lungs of the rMS group were localised, while those in the control group were extensive.CONCLUSION:
rMS had immunotherapeutic effects associated with a switch to the Th1 response and the antibacterial activity of GLS.
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Base de dados:
MEDLINE
Assunto principal:
Tuberculose Pulmonar
/
Tuberculose Esplênica
/
Antígenos de Diferenciação de Linfócitos T
/
Interleucina-12
/
Mycobacterium smegmatis
/
Vacinas contra a Tuberculose
/
Mycobacterium tuberculosis
Tipo de estudo:
Prognostic_studies
Limite:
Animals
/
Female
/
Humans
Idioma:
En
Ano de publicação:
2009
Tipo de documento:
Article