Analysis of tissue-specific gene expression using laser capture microdissection.
Methods Mol Biol
; 585: 183-92, 2010.
Article
em En
| MEDLINE
| ID: mdl-19908005
ABSTRACT
Epithelial tissues exhibit optimal conditions for studying cellular differentiation since the differentiation status of a single cell can be determined by its distance to the basal membrane. For that reason Laser Capture Microdissection (LCM) may serve as a perfect tool to compare the characteristics of cells that have been collected from different strata of the epithelium. However, as cell boundaries are not visible in untreated tissue sections, samples have to be stained to allow for sufficient structural orientation. This usually results in a considerable reduction of RNA content in the dissected specimen. To circumvent this problem, we have established a modified hematoxylin/eosin staining protocol that concurrently allows visualization of important structures and the subsequent isolation of sufficient RNA amounts to be used for linear amplification and quantitative analyses.
Texto completo:
1
Base de dados:
MEDLINE
Assunto principal:
Expressão Gênica
/
Perfilação da Expressão Gênica
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Microdissecção
/
Lasers
Idioma:
En
Ano de publicação:
2010
Tipo de documento:
Article